The TSB branch in Milton Keynes will not be amongst the 70 UK branches closing this year as customers shift to online banking.
TSB said 150 employees would be affected but all were to be offered alternative roles at the business.
TSB said that in recent years it had seen a "significant decrease in branch use" and that it saw "no prospect of branch transactions returning to pre-COVID levels".
The latest announcement follows a strategy set out by the bank two years ago to scale back its high street network and invest in digital services.
Robin Bulloch, TSB's chief customer officer, said: "Closing branches is an incredibly difficult decision to take, but we have to respond to the changes in the way people bank and provide the right mix of services for all our customers now and into the future."
You can find a full list of the branches affected here.
Additional content by Sky News
